Atherosclerosis is one of the biggest problems of cardiovascular disorders and has been studied for the past 25 years. In Indonesia, the increase of coronary heart disease prevalence seems to be influenced by the increase in socio-economic levels accompanied by a change in diet from foods that contain lots of fiber to foods that contain lots of fat. It can be seen from the results of the 1986 Household Health Survey on ischemic heart disease conducted in seven provinces in Indonesia with a significant raise in the 45-54 year age group of 174.6 per 100,000 population. In the older age group, 55 years and over, the prevalence reached 461.9 per 100,000 population. The WHO in 1990 estimated that the leading cause of death per year was cardiovascular disease, which is 12 million per year globally.
The purpose of this research is to determine the effect of black tea administration on serum adiponectin levels in male Wistar rats fed with atherogenic diet (AD). This research was designed as a true experimental design that used 20 mice and divided them into four groups as follows: P1 (AD 3 months); P2, P3, and P4 (administration of black tea extract 3 months AD + with doses of 0.015gr / day, 0.03gr / day, 0.045gr / day, respectively). Serum adiponectin levels were measured by the ELISA method.
This research showed shows that there is a tendency for adiponectin levels to increase with increasing doses. However, serum adiponectin levels in the AD only-group (380.6ng / mL) was not significantly different from the treatment group (386.93ng / mL, 404.27ng / mL, and 416.6ng / mL in P2, P3, and P4 respectively) (ANOVA, p = 0.352). Furthermore, there was no significant association between black tea dose and adiponectin levels (Pearson correlation test, p = 0.073, r = 0.373). We concluded that black tea extract increased serum adiponectin levels insignificantly in the atherogenic diet rats.
The results of this research showed a positive correlation, meaning that the higher the dose of addition of black tea, the higher the results of measured adiponectin levels. Furthermore, the induction of a high-fat diet increased the size of inguinal fat cells and, when compared to other studies, administration of brewed black tea extract decreased the size of fat cells. However, based on the ANOVA test, the increase in adiponectin levels was not significant. It can be caused by various factors, including inadequate maintenance of rats which can trigger stress. The lab rat rearing room should have a relative humidity of 55 ± 10%, the temperature ranges from 23-27 ˚ C, the duration of exposure is 12 hours, not noisy, and the air is maintained, comfortable, room cleanliness must always be maintained, drink bottles must be cleaned twice a week. In conducting the research, those aspects may get less attention so some rats got stressed.
Based on the results of the research regarding the effect of giving black tea extract on adiponectin levels, it can be concluded that black tea extract does not significantly increase serum adiponectin levels in atherogenic diet rats. Further research is needed on the protective effect of black tea against atherosclerosis in a more real (in vivo) sample so that the effect of black tea can be measured accurately. Further pharmacologically and mathematically research is needed to determine the optimal dose of black tea. A research on the regulation of adiponectin production with a more definite path is needed.
Author: Meity Ardiana
The full article:
