Toxo Kit, New Generation of Toxoplasma Test

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Toxo Kit, Toxoplasma Diagnostic Product made by FKH UNAIR lecturers (Photo: Faridah Hariani)

UNAIR NEWS –  A team of lecturers from Faculty of Veterinary Medicine (FKH) Universitas Airlangga has made another innovation. This time, the team consists of Prof. Dr. Lucia Tri Suwanti, Dr. Mufasirin, Prof. Dr.  Suwarno, Prof. Meles, Dr. Hani Plumerastuti and a partner from Mataram, drs. Zainul, has made and developed a diagnostic kit for toxoplasma. It is called “Toxo Kit”. It was firstly developed and studied in 2014, and it has been in a development phase since then.

“The development of this kit was because of the need of diagnostic tools, such as for toxoplasma,” said Mufasirin. “All cost and research funding was from Kemenristek Dikti, in order to improve the quality of the lecturers,” he added.

All this time, the testing of toxoplasma uses a diagnosis kit called “Uji ELISA (Enzyme-Linked Immunosorbent Assay)”. But to have the result, ELISA testing takes too long time about two days while with Tox Kit we just need about 10 to 15 minutes.

This working method of this Toxo Kit is almost the same with Test pack or pregnancy test. First the some blood is extracted then sedimented and dropped to Toxo Kit. After a while we will get the result. If positive, then there will be two lines, and if negative, there will be only one line.

Toxo Kit contains antigen which works to ctach material in the sample or antibody. Then it is equipped with reactive signal. If the sample is positive, the reactive signal will change color. Toxo Kit has sensitivity or accuracy rate 73.5% and specificity 66.7%.

“It still can’t be compared with the conventional toxolasma testing, and the standard is ELISA, but Toxo Kit can be used as an alternative diagnosis of toxoplasma,” said Mufasirin.

With Toxo Kit, Mufasirin and his team hoped that it can help the community in diagnosing toxoplasma which is deemed costly and lengthy. In the future, Mufasirin and team will try to optimize the kit by improving the accuracy and specificity.

“We have communicated with one of our chemical manufacturers to produce the kit. They have their own standards for something to be manufactured. Therefore we will improve the quality so it won’t be much different when it is mass produced,” said Vice Dean II FKH UNAIR.

Moreover, Mufasirin and team have planned to develop the kit to be a multi-functions kit, not only detect Immuniglobulin G, but also Immunoglobulin M. So it can detect more than one disease. (*)

Author : Faridah Hari

Editor : Dilan Salsabila

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