UNAIR NEWS – From year to year, the demand for orthodontic treatment in the adult female population is increasing. During orthodontic treatment, do not kneel the possibility they are pregnant. Pregnancy will cause significant physiological and anatomic changes in the mother’s body.
In his research on “The Role of Vitamin D for Tooth Movement”, Prof. Dr. drg., Sp.Ort. (K), said that Vitamin D is needed during pregnancy, which can be obtained by oral route or skin exposure to sunlight.
It is known that the level of vitamin D deficiency is high in Southeast Asia, with a prevalence of 6-70% of the Asian population. Vitamin D can improve endothelial cell function and angiogenesis through the VEGF dependent pathway.
“Angiogenesis will ensure adequate blood flow to provide an adequate supply of nutrients and oxygen,” he added.
According to Prof. Ida Bagus, the research is to analyze the effect of vitamin D on the expression of vascular endothelial growth factor (VGEF) and the amount of angiogenesis during orthodontic tooth movement (OTM) in pregnant Wistar rats (Rattus norvegicus).
Prof. Ida Bagus said that the methodology used was experimental with a post-test only control group design. Furthermore, Lameshow sample size formula was used with the random sampling method.
“Wistar rats were divided into two control groups of the seventh day and fourteenth day and two groups of experiments, seventh experiment and fourteenth experiment. Their pregnancy was synchronized, the strength of the closed coil spring 30g / mm was given in between incisors and the first left maxillary molar. “Vitamin D (0.2 mg/kg) is given every three days until the seventh and fourteenth experiments,” he said.
The maxillary bones of all groups were extracted, and the number of angiogenesis was examined by hematoxylin-eosin staining, and VEGF expression was examined by immunohistochemical staining. Besides, we also conducted Mann-Whitney and analysis of variance with Tukey HSD post-hoc test with a standard deviation (p, red) of less than 0.05 based on the Levene test and the Kolmogorov-Smirnov test results found a standard deviation of more than 0.05.
The results showed the lowest angiogenesis rate found in the seventh-day control group was (11.11 ± 5.95), and the highest was found on the fourteenth day (23.83 ± 7.10). By a significant difference obtained between the seventh day and the fourteenth experiment (p = 0.003, p
“The lowest VEGF expression was found in the fourteenth experiment (3.09 ± 1.14) while the highest was found in the fourteenth-day control (6.26 ± 2.39). There is no significant difference between each group (p> 0.05), “he explained.
He revealed that vitamin D does not significantly increase VEGF expression and the amount of angiogenesis in OTM in pregnant mice. (*)
Author: Asthesia Dhea Cantika
Editor: Nuri Hermawan
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